Plant
material
Plant was collected from southern kordofan state 2020
and sample was identified and authenticated by the taxonomists of the medicinal
and aromatic plant and traditional medicine research institute (MAPTMR)
Khartoum, Sudan. They were washed and then air dried under light exposure
(27C-30C) for 14 days.
Plant
extraction
The extraction was carry out according to protocol of
WHO 1998. And successive extraction method.
Phytochemical
screening
Phytochemical was carry out and show the present of
tannin by addition of ferric chloride
reagent to the filtrate was given blue colour indicate the presence of tannin
Alkaloids by addition of few drop of Dragendorffs reagent turbidity was taken as
indicative of presence of alkaloids, flavonoids indicated by addition of
magnesium metal followed by the addition of few drop of conc. HCL the red
colour was inductive the present of
Flavonoids, saponins content was determined by boiling 1 g powder in 10
ml distilled water for 15 min and after cooling the extract was shaken vigorously
to record froth formation, steroid was determined by dissolved the extract by
chloroform and faltered H2SO4 was added to filtered to
form lower layer reddish brown colour steroidal ring was appear, carbohydrate
was determined by Mulish test, reduced sugar was determined by Fehling
reagent.
Proximate
analysis
Proximate analysis was carry out to show presence of
fat the sample was hydrolyzed by hydrochloric acid at 70-80C.protein,
if any, can be dissolved in acid, cured fat manually extracted by diethyl and
petroleum ether .the solvent was removed by evaporation and the oil residue
dried and weighted, Moisture e method was based on drying sample under control
temperature until constant weigh is obtained, Ash method was involve oxidation
of all organic matter by incineration in a furnace at specific temp less than
(550C) Ashing above 650C volatilities inorganic salt like
alkali chloride and a portion of ash
fused and enclosed some carbon , preventing them from benign ignited. The
residue left after incineration is the Ash
content of the sample, protein method was based on digestion of protein
and organic food with sulfuric acid in catalyst to release nitrogen from
protein ammonium gas was liberated upon the addition of excess alkali and was
distilled in to a boric acid solution to form ammonium borate complex the
ammonium liberated was tittered with
standardized HCL the amount of nitrogen was determined from Mg equivalent to
acid uses crude protein was determined by multiplied nitrogen content with
conversion factor to food matrix and fiber method was extracted by 2g ether then precipitate was formed and
transfer to digestive flask unit with addition to espstous, 200mg of H2SO4
was added digestion flask was connected to condenser then boiled, funnel was
fixed with piece of cloth and then added regular a hot water to wash H2SO4,
then NaoH was added to the cloth precipitate until the alkali is removed.
Method
of antimicrobial activity
The antimicrobial test was performed using agar
diffusion method. The test microorganism were incubated on nutrient agar plate
and separate uniformly using sterile glass separator. Wells of 5mm in diameter
were made on the nutrient agar using sterile cork borer. The cute agar disks
were carefully removed by the use of forceps sterilized by flaming then the
extract was added to plate. The plate is allowed to stand for one hour at room
temperature for diffusion of the substance to proceed before the growth of
microorganism commenced. The plate were incubated at 37oC for 24 h.
the zones of inhibition were then recorded.